Interactive spectrum Widely used in both research and industry, infrared spectroscopy is a simple and reliable technique used for a variety of measurements and in quality control. It is especially useful in forensic science both in criminal and civil cases. Spectrometers are now small, and can be easily transported, even for use in field trials.
By Carl Saxton Mass Spectrometry, also referred to as mass spec, is an analytical technique becoming increasingly important in bioscience research. This article will introduce you to this technique, how it works, and how it could be useful to you in your research.
In a nutshell, mass spectrometry accurately measures the mass of different molecules within a sample. Even large biomolecules like proteins are identifiable by mass, which means that biologists can perform some very interesting experiments using mass spectroscopy, potentially adding a new dimension to your research.
Yes, if you want to: Identify molecules in a mixture Detect impurities in a sample Analyze a purified protein Study the protein content of a sample of cells How Does Mass Spec Work? There are three key stages to the spectrometer: Then, an electron beam bombards the vapors, which converts the vapors to ions.
Because mass spectroscopy measures the mass of charged particles, only ions will be detected, and neutral molecules will not be seen. Ions are created by giving electrons to a molecule producing a negatively charged ion or taking electrons away from a molecule producing a positively charged ion.
Next, the ions are sorted according to mass in two stages — acceleration and deflection. The positive ions created in the ionization stage accelerate towards negative plates at a speed dependent on their mass.
In other words, lighter molecules move quicker than heavier ones. So, ions of different mass travel through the spectrometer at different speeds. The output will look something like in Figure 1. Sample spectrum produced by mass spectrometer.
Is Mass Spectroscopy Worth It? Mass spectroscopy provides accurate weight measurements for your bio- or other molecules, which can be used to: Give a good estimate on the purity of the sample i. With the development of this tool, researchers can now feasibly begin defining biochemical pathways on a kinetic basis, which will help us understand the mechanistic response of cells to changing environments.
Peptides, Hospitals, Drugs and Crime Scenes: Practical Uses of Mass Spectroscopy Drug companies and researchers are using the technique for drug discovery, for gaining information on drug metabolism and for pharmacokinetic studies.
These uses also make mass spectroscopy when coupled with other analytical techniques a powerful tool in forensic analysis. If you feel that mass spectroscopy is something that could be of use in your research, check whether your department or college has mass spectrometers available for use.
Training will always be provided for these services so ask around. While the number of uses is large, the instrument itself is not —normally the machine can fit on a bench top.
As many a chemist will testify, this is often an issue with communal mass spectrometers—especially if the user before you did not the column properly.
Want to Know More? There are various ways of producing the ion required, and the method chosen depends on the nature of the sample molecule. You can find out more information in the references below.In vivo magnetic resonance spectroscopy (MRS) is a specialized technique associated with magnetic resonance imaging (MRI)..
Magnetic resonance spectroscopy (MRS), also known as nuclear magnetic resonance (NMR) spectroscopy, is a non-invasive, ionizing-radiation-free analytical technique that has been used to study metabolic .
Highlights • Review of current approaches to PAT for proteins in DSP. • The review focuses on spectroscopy as an emerging tool for PAT. • An introduction . Microorganisms play an important role in food industry. As already discussed in the earlier article Contributions of Microbiology in Food Industry, they are used in production of various food products, and are also responsible for food spoilage thereby causing intoxication and diseases.
Microbial contamination o. Raman spectroscopy is one of the vibrational spectroscopic techniques used to provide information on molecular vibrations and crystal structures. This introduction explains the principles and practice of Raman spectroscopy. ABSTRACT.
This paper intends to review the basic theory of Near Infrared (NIR) Spectroscopy and its applications in the field of Analytical Science. Countries around the world are deploying radiation portal monitor (RPM) systems and other radiation detection instrumentation to interdict the illegal shipment of radioactive material crossing international borders.
These efforts include deployments in the U.S. and in a number of other countries by.